If initialization has in the point estimator has been reduced to a negligible level, the method of independent replications can be used to estimate point estimation variability and to construct a confidance interval.

If significant bias remains in the point estimator and a large number of replication are used to reduce point estimator variabilities the result confidence interval can be misleading.

The bias is not affected by the number of replications R, but by deleting more data (i.e increasing To) or extending the length of each run (i.e increasing TE)

Giving the run length, the number of replications should be as many as possible. Kelton is 1986 established that there is little valueto run more than 2S replications. So if time is available, make the simulation longer, instead of making more replication.